MIRI® Time-Lapse Incubator

The MIRI® Time-Lapse Incubator is a multiroom incubator with a built-in camera and microscope. Designed and manufactured in EU, the MIRI® Time-Lapse Incubator provides high quality time-lapse images of embryos developing in "real-time" without having to remove the embryos from the safety of the incubation chamber for manual microscopy. Time-lapse embryo monitoring provides detailed morphokinetic data throughout embryo development, which is not available on routine spot microscopic evaluation. This allows all important events to be observed, helping to identify healthy embryos with the highest probability of implantation, with the aim of achieving higher pregnancy rates.

For Time-Lapse & Animal IVF

Features

Completely Independent Chambers

Time-Lapse Monitoring

Heated Lid

Fast Temperature and Gas Recovery

Easy External Gas and Temperature Validation

Superior Gas System

User Friendly Interface

MIRI® TL Datalogger

The CultureCoin®

Continues pH Monitoring

MIRI® TL Viewer Software and MIRI® TL Server

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Product Details Product Features Introduction Simple guide on how to operate the MIRI® TL Ordering Operation Literature & Resources

Completely Independent Chambers

6 (TL6) / 12 (TL12) independent chambers with precise temperature and gas regulation: Opening any chamber does not affect the environment in other chambers.
Completely Independent Chambers
Time-Lapse Monitoring

Time-Lapse Monitoring

Time-lapse video generated from images allows objective and reliable scoring and annotation of embryos for better prediction of future developmental and implantation potential.

Heated Lid

Excellent uniformity between the top and bottom lid.Heated chamber lids prevent condensation, enhance temperature regulation/recovery, and ensure temperature uniformity between the top and bottom of the chamber.
Heated Lid

Fast Temperature and Gas Recovery

  • Temperature recovery <1 min
  • Gas recovery <3 mins.

Easy External Gas and Temperature Validation

Gas sampling ports for each chamber and independent PT-1000 sensors allow external quality evaluation of gas levels and temperature in each chamber.

Superior Gas System

  • Built-in gas mixer for tri-gas, an advanced gas regulation system, and HEPA-VOC filtered recirculation allows precise gas level control and a lower gas consumption.
  • UV-sterilization of recirculating gas ensures biological safety of embryos.
User Friendly Interface

User Friendly Interface

Touchscreen control panel with intuitive menu to manage configuration of the device

MIRI® TL Datalogger

Continually monitors and documents gas flow, gas concentration and pressure, temperature regulation.
Time-Lapse Monitoring

The CultureCoin®

Specially designed culture dish, the CultureCoin, allows each chamber to culture 14 embryos, giving a total capacity of 84 embryos for the TL6 and 168 embryos for the TL12.

Continues pH Monitoring

Optional SAFE Sens integration for continuous pH monitoring without opening the chamber.
Continues pH Monitoring

MIRI® TL Viewer Software and MIRI® TL Server

  • Exponentially increase image and data storage capacity by adding the MIRI® TL Data Server.
  • Combine MIRIM® TL Viewer software platform to review, annotate and compare morphokinetic parameters of embryos, as well as export data for retrospective analysis.

MIRI® TL Viewer Software and MIRI® TL Server

Introduction

Timelapse Technology

Time-lapse Technology:
Addressing IVF Risks

Time-lapse technology is a current system for the culture and imaging of embryos in vitro in the practice of Clinical Embryology. Risks associated with In Vitro fertilization (IVF) like multiple pregnancies and the related mother and neonate death rate has increased the past few years.

While more stringent policies on embryo transfer have eradicated some of these risks, the impact on the occurrence of twins remains to be a concern. It is said that a twin gestation can be evaded through a single embryo transfer only. Conversely, the conventional way of selecting the embryo’s morphology is not sufficiently and effectively analytical and predictive to permit routine single transfer of embryo; hence, novel screening tools are necessary.

Through the use of a time-lapse incubator, a continuous, non-invasive way of monitoring embryos is possible without the removal of the embryo from maintained, optimum culture conditions. With this, an IVF practitioner is able to observe information concerning cleavage patterns, morphologic deviations, and the dynamics of embryo development. The constant monitoring could support the identification of embryos with higher/ better implantation potential. Such technologic advancement allows the unbiased selection of the embryo(s) for transfer.
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A Closer Look at the MIRI® TL Incubator

Esco Medical’s MIRI® TL Incubator boasts off features that provides stable environment, improved embryo selection, improved quality management, and a better quality control assay in the IVF laboratory.

  • A. Unique Incubation Environment: With 6/ 12 individual chambers wherein one patient can be designated per chamber to avoid cross-contamination. The time-lapse in a chamber continues undisturbed even if the neighboring chambers are opened. Also, one can set individual temperature set-point for each chamber.

  • B. Excellent Temperature Uniformity and Regulation: It has one independent temperature sensor for external validation (MIRI® GA), controlling each chamber with 3 individual sensors that gives an extremely accurate temperature.

  • C. The MIRI® TL has a built-in tablet that has 128 Gb space capacity to store time-lapse images in the event of a faulty connection between the MIRI® TL and the Viewer Software.

  • D. Solid Validation System: Each chamber has built-in PT1000 validation sensor that is separate from system circuitry for connection of external temperature measuring device. Six (6) gas sampling ports allow sampling of gases easily via an external gas analyzer. It even has a built-in pH measurement system.

  • E. Excellent Recovery Time: The MIRI® TL has excellent recovery rates:
    • Gas – less than 3 minutes
    • Temp – less than a minute
  • F. Built-in gas mixer: The MIRI® TL has a built-in gas mixer which allows users to change culture media without ordering/ trying costly pre-mixed gas. One can use either premixed gas or pure gas.

  • G. Other excellent features include: Assist Function in the software registers the first three divisions automatically. This is a unique feature of the MIRI® TL User-friendliness of MIRI® TL software provides flexibility and versatility to the embryologists to add or edit events or sub-events. A special dish for MIRI® TL use only, called the CultureCoin®, can accommodate 14 embryos. Embryos are also easier to load using the CultureCoin®.

The Silent Embryo Hypotesis

The Silent Embryo Hypothesis

Baumann et al said that “the Quiet Embryo Hypothesis proposes that viable preimplantation embryos operate at metabolite or nutrient turnover rates distributed within lower ranges than those of their less viable counterparts”.

The MIRI® TL is a multi-room incubator with a built-in microscope and camera that continuously captures images of embryo developments until the day of transfer without any disturbance. This enables a detailed scoring of the embryos cultured, for better prediction of developmental and implantation potential.

Conventional assessment of embryo selection in IVF is often limited to static observations at pre-defined time intervals, which requires the embryo to be taken out from the incubator. With the MIRI® TL, the dynamic process of embryo development can be observed without disturbances to culture conditions. Hence, this supports the Silent Embryo Hypothesis, which observes that embryos develop better if less disturbed.

Simple guide on how to operate the MIRI® TL

I. CultureCoin® Preparation

  • 1. Preparation of the CultureCoin®, the media, and oil must be done a day before loading the embryos.
  • 2. Before loading the CultureCoin® with the media and oil, see to it that you have pre-equilibrated the following inside a humidified incubator:
    • The culture media and the CultureCoin® for 4 to 6 hours
    • The Overlay oil for 1 to 2 days.
  • 3. Always ensure that you work inside an IVF Workstation. Load the CultureCoin® with the 25ul of media under the microscope. (Do not inject the media directly on the bottom of the well.)
  • 4. Fill it on its wall and remove any visible bubbles from the media with a stripper tip.
  • 5. Cover the media with 3 - 3.5 ml of pre-heated oil.
  • 6. Put the CultureCoin® with the lid inside an incubator and leave it for more than 12 hours for equilibration.
CultureCoin® - Embryo Culture Dish for the MIRI® TL

II. Loading of the Embryos

  • 1. After equilibration, check for air bubbles in the culture wells. Remove any bubbles with a stripper tip.
  • 2. Load the embryos into the center of the filled microwells with a plastic stripper tip.
  • 3. Place the embryo at the center of the well.
  • 4. Once done, carefully put the embryo loaded CultureCoin® inside an unoccupied MIRI® TL chamber.
  • 5. Select patient then select/deselect active positions and start Time-Lapse when ready.

III. Embryo Annotation

  • 1. On the MIRI® TL Viewer Home Screen, select “TimeLapses” and look for the patient to open time-lapse videos. A revolver view with the time-lapse video of each of the14 wells in the CultureCoin® will be displayed.
  • 2. Select an embryo to annotate. The embryo will be maximized at the center. The annotation system is structured around the “events” that are located on the left of the wheel.
  • 3. You can also use the ASSIST tool to automatically annotate the first 4 cleavage events.
MIRI TL 6 And viewer

IV. Selecting the most Viable Embryos

Selecting the most Viable Embryos
  • 1. The MIRI® TL Viewer software is equipped with sophisticated tools to help you evaluate and select the most viable embryo after completely annotating all embryos.
  • 2. The Compare Tool will let you do a side-by-side comparison of two embryos. Two embryo videos can be played independently or linked together for comparison.
  • 3. The “Summary” tool will allow you to compare all embryos in an informative and graphical way. It will let you compare embryo developmental timings and which one develops closely with the ideal timings.
  • 4. Once the selection process has been completed or a decision has been made, click the green check icon in the upper left corner. After clicking it, a drop-down list of the desired status will appear.
  • 5. Assign a corresponding color that indicates the decision in each well: Green for “transfer”, Red for “discard”, and Blue for “freeze”. The colored ring will appear around the well to indicate the decision made. You can also click the “Dish Map” icon to visually map the decision that has been made.

Ordering Operation

Item Code Model Code Description
Unit    
2070091 MRI-TL-MN-6C-8 MIRI® Time-Lapse Incubator, Mini, 6 Chambers, 230 V, 50/60 Hz
2070092 MRI-TL-MN-6C-9 MIRI® Time-Lapse Incubator, Mini, 6 Chambers, 115 V, 50/60 Hz
2070098 MRI-TL-MN-6C-SS-8 MIRI® Time-Lapse Incubator, Mini, 6 chambers with SAFE Sens, 230 V, 60/60 Hz
2070099 MRI-TL-MN-6C-SS-9 MIRI® Time-Lapse Incubator, Mini, 6 chambers with SAFE Sens, 115 V, 60/60 Hz
2070100 MRI-TL12C-8 MIRI® Time-Lapse Incubator, 12 Chambers, 230 V, 50/60 Hz
2070101 MRI-TL12C-9 MIRI® Time-Lapse Incubator, 12 Chambers, 115 V, 50/60 Hz
2070092 2070092 2070092
Accessories    
1320011 MRA-1007 HEPA + VOC filter (to be replaced every 3 months)
1320088 MRI-CC CultureCoin for Time-Lapse of 14 embryos (25 pcs. per pack)
1320045 MRI-GA MIRI® GA CO2 /O2 & Temperature Validation Unit, 115V/ 230V
TBA TBA MIRI® Time-Lapse Incubator, 12 chambers with SAFE Sen s, 230 V, 60/60 Hz
TBA TBA MIRI® Time-Lapse Incubator, 12 chambers with SAFE Sens, 115 V, 60/60 Hz

Literature & Resources