MIRI® II Multiroom Incubator

The Esco Medical MIRI® II-12 incubator is intended to be used to provide a stable culture environment at or near body temperature and CO2/N2 gases for the development of gametes and embryos during in vitro fertilization (IVF) / assisted reproduction technology (ART) treatments.

MIRI® II Multiroom Incubator dedicates one chamber for one patient. It has 12 chambers that are completely independent of each other. Any disruption on one chamber (e.g. temperature drop after opening the lid) has zero impact on the rest of the system. It has a touchscreen PC to control and monitor important parameters (temperature, gas concentration) simultaneously and give visual and audible alarm to signal critical conditions.

Key Features:

  • It has multiple independent chambers with very stable environments, allowing embryologists to culture embryos from individual patients in individual chambers.

  • It has a reliable gas mixing system that allows gas-phase flexibility. The gas mixer of MIRI® II gives total control over CO2 and O2 concentration levels while also giving flexibility over what gas input is desired.

  • The heated lid prevents condensation and improves temperature regulation and recovery enhancing uniformity within each chamber. The heated bottom provides direct heat transfer to the culture dishes for stable heat regulation.

  • One important benefit is the speed of recovering temperature and gas parameters after opening a chamber.

  • Allows users to control and monitor important parameters (temperature, gas concentration) simultaneously and gives visual and audible alarms to signal critical conditions. It has a large display that can be easily seen from a distance.
    MIRI II heating plates

  • A removable heat optimization plate in each compartment can be removed and cleaned; this part serves as the main holding area for samples it should be the highest priority to keep it clean.

  • The MIRI® II can be connected to an external device such as the Esco MIRI® GA for gas and temperature validation.

To ensure maximum performance, the system of MIRI® II-12 has 24 completely separate PID temperature controllers. They control and regulate the temperature in culture chambers and lids. Compartments do not affect each other's temperatures in any way. The top and the bottom of each compartment are separated with a PET layer so that the lid temperature would not affect the bottom. For validation purposes, each compartment has a PT-1000 sensor built-in. The circuitry is separated from the unit's electronics, so it remains a genuinely separate validation system.

The incubator needs 100% CO2 and 100% N2 to control CO2 and O2 concentrations in the culture chambers. A dual-beam infra-red CO2 sensor with extremely low drift rates controls the CO2 level. A chemical, medical-grade oxygen sensor controls the level of O2.

Gas recovery time is less than 3 minutes after opening the lid. MIRI® II-12 incubator is fitted with 12 gas sample ports that allow the user to validate gas concentration by sampling gas from the individual compartment.

The incubator also features a recirculated gas system where gas is continuously put into the compartment and taken out at the same rate. Gas is cleaned via 254 nm UVC light with direct gas contact between the bulb and gas, then through a VOC filter and a HEPA filter. The UVC light has filters that inhibit any 185 nm radiation that would produce dangerous ozone. The VOC filter is located under the UVC light. The complete gas repletion in the system takes less than 5 minutes.


The incubator has a complete gas control system that consists of the following:



  1. Pressure regulator (preventing dangerous gas pressure problems)

  2. Gas flow sensors (actual consumption can be accumulated)

  3. Gas pressure sensors (allows the user to know the pressure and the variation can be logged to avoid dangerous conditions)

  4. Gas filters (to prevent valve problems)


Petri dish location: Found in a compartment that is easy to reach and safe because of the compartment numbering and the ability to write on the white lid with a pen.

The software: It runs on the built-in touchscreen in MIRI® II-12 version. The software contains logging functions for long-term data logging and storage.

USB module: This enables the quality control data to be transferred for off-site evaluation. The performance of such allows the manufacturer to provide a valuable service to the customers.

A pH sensor port: This is part of the DAQ package wherein the user can plug any standard BNC pH probe into the unit and measure the pH in the samples at will.


Important Parts of MIRI II


GENERAL SPECIFICATIONS of MIRI II


Technical specifications

MIRI® II-12

Overall dimensions (W x D x H)

740 x 575 x 215 mm

Weight

47 kg

Material

Mild steel / Aluminum / PET / Stainless steel

Power supply

115V 60Hz or 230V 50Hz

Power consumption

500W

Temperature range

25.0 - 40.0 °C

Gas consumption (CO2)

< 2 liters per hour

Gas consumption (N2)

< 12 liters per hour

CO2 range

3.0% - 10.0%

O2 range

5.0% - 10.0%

CO2 gas pressure (input)

0.4 - 0.6 bar (5.80 - 8.70 PSI)

N2 gas pressure (input)

0.4 - 0.6 bar (5.80 - 8.70 PSI)

Alarms

Audible and visible for out-of-range temperature, gas concentration and gas pressure.

The Esco Lifesciences Group