CultureCoin® - Embryo Culture Dish for the MIRI® TL

CultureCoin® - Embryo Culture Dish for the MIRI® TL

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Workflow Categories
  • RFID Witnessing and Traceability System
  • Media Storage
  • Reagent Pre-equilibration
  • Oocyte Retrieval
  • Sperm Washing
  • IUI
  • Fertilization
  • Embryo Culture
  • Embryo Assessment & Selection
  • Cryopreservation
  • Embryo Thawing
  • Embryo Transfer
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CultureCoin® - Embryo Culture Dish for the MIRI® TL

CultureCoin® - Embryo Culture Dish for the MIRI® TL

The Esco Medical CultureCoin® is an IVF culture dish designed specifically for the MIRI® TL. The MIRI® TL is Esco Medical’s time-lapse incubator that captures real-time images of developing embryos which can be viewed as time-lapse videos.The CultureCoin® is a single-use, gamma-sterilized culture dish that has oxygen plasma-treated surfaces for effective prevention of bubble formation. Its ergonomic design makes it easy for embryologists to load a total of 14 embryos while being able to safely and securely handle them inside the incubator. This culture well features a 300µm, optically clear area for embryo placement which is optimized for microscopy.

Workflow Categories

Embryo Culture Embryo Culture

Features

Numbered culture wells (1-14)

Optional Lids

pH reservoir

Flat bottom

Writeable area and lid

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Product Details Parts of the Parts of the CultureCoin® General Specifications Ordering Information Literature & Resources

Parts of the CultureCoin®

Parts of the CultureCoin®

General Specifications of the CultureCoin®

Overall dimensions (D x H) ⌀ 71 x 10 mm
Weight 13.8 grams
Material Styrene Methyl Methacrylate (SMMA)
Temperature range 28.7 ‐ 40.0 °C
CO2 Incubation Range 1.9 ‐ 10%
O2 Incubation Range 4.9 ‐ 20.0%
Sterilization method Gamma irradiation
Shelf life 2 years
Biocompatibility tests
  1. Mouse Embryo Assay (MEA) test with thawed 1-cell mouse embryos. Acceptance criteria: at least 80% of embryos developed to the blastocyst stage.
  2. Limulus Amebocyte Lysate (LAL) test. Acceptance criteria: < 20 EU/device.

How to use the CultureCoin®

I. CultureCoin® Preparation

  1. Leave the dishes with the lids on for off-gassing in an aseptic, humidified environment for at least 1 hour.

    Note: Warm the dishes on a heated stage or in an incubator before filling them with media.

  2. Prepare the media and oil, then place them in a humidified incubator for 4 to 6 hours for pre-warming and equilibration.
  3. Fill all culture wells with approximately 25 µl of pre-equilibrated culture media in an aseptic environment.
  4. Cover the culture media with a confluent layer of suitable mineral or paraffin oil. The recommended amount is 6 ml. Do not overfill.

    Note: Filling the washing wells is optional. One washing well holds approximately 23 µl of solution. The wells can be used for cell manipulation, washing media, or replacement media.

  5. Check the CultureCoin® under a microscope and remove any visible air bubbles with a stripper tip.
  6. Put the lid on the CultureCoin® dish and leave it to equilibrate in a CO₂ incubator for at least 4 hours. A humidified incubator is preferable.

II. Loading the Embryos

  1. After equilibration, check the culture wells for air bubbles. Remove any bubbles with a stripper tip.
  2. Load the embryos into the microwells with a plastic stripper tip.

    Note: Make sure the embryos are positioned precisely in the centre of the culture well and not on the side slope.
    Optional: Fill the pH reservoir with culture media (recommended volume: 350 µl) and cover it with an oil overlay (recommended volume: 150 µl).

  3. Once completed, place the CultureCoin® in the MIRI® TL family multiroom IVF incubator.

Ordering Information

CultureCoin®
Item Code Model Code Description
1320088 MRI-CC CultureCoin® for Time-Lapse of 14 embryos (25 pcs. per pack)

Literature & Resources