Workflow Categories
  • RFID Witnessing and Traceability System
  • Media Storage
  • Reagent Pre-equilibration
  • Oocyte Retrieval
  • Sperm Washing
  • IUI
  • Fertilization
  • Embryo Culture
  • Embryo Assessment & Selection
  • Cryopreservation
  • Embryo Thawing
  • Embryo Transfer
Products
Featured Products
View All Products

Time Lapse Imaging and Morphokinetics Parameters

By Esco Medical, 07 May, 2015

Esco MIRI® TL is a continous monitoring system of embryo development through multiple, detailed image acquisition without compromising embryo culture condition. Traditional morphological assessment on embryo is limited to daily views at fixed time points and embryologist could potentially lose information during embryo development.

 

Time lapse imaging system which integrated into bench top incubators provide:

 

  1. Un-interrupted culture condition with separate heating chambers. Temperature and pH are crucial parameters for an optimal culture condition
  2. Identification of anomalies
  3. Multiple, detailed time lapse images of embryos
  4. Data for precise timing of a specific event through annotation procedures
  5. Improvement of documentation procedures
  6. Develop criteria’s for selection/de-selection and transferring most viable embryo(s)

 

Morphokinetics parameters are important information for embryologist to decide the most viable embryo(s) for transfer. Some of these include:

 

  1. PN formation. Assessment of appearance or fading of pronuclei. Time-lapse is able to improve assessment accuracy by knowing the exact timing of the events.
  2. Cell cleavage. Observe normal or abnormal cleavage pattern or cleavage rate.
  3. Symmetry. Embryo symmetrical shape during embryo development
  4. Fragmentation. Reabsorption of fragmentation only being discovered after time lapse being introduced.
  5. Multinucleation of the embryo 
  6. Cell compaction rate and exact timing of event occurred.
Previous Resources Are We on the Same Page? A Guide to Time-Lapse Nomenclature and Annotation

View Other Resources

How to implement time-lapse system in daily IVF practice

Prof. Dr. Thomas Ebner, Kepler University, Linz, Austria Since the birth of Louise Brown (Steptoe and Edward, 1978) the field of assisted reproduction underwent fundamental changes in fertilization techniques (Palermo et al., 1992), controlled ovarian

Read More

Why Maintaining CO2 Balance is Important

A careful manipulation of gamete and embryos has always been an important element of embryology as it contributes to the overall success of ART. The regulation of CO2 concentration all throughout the IVF process from egg retrieval to preparation and fertilization up to incubation is of paramount importance as this gas helps regulate the pH of the medium.

Read More

What are you missing without Time-Lapse?

Diana Stein, embryologist for over 30 years and Lab Director of Laniado Hospital-Sanz Medical Center (Israel) and a MIRI® Time-Lapse user, had an initial impression after a quick look of the embryo status morning of Day 2 to transfer the embryo on the right, based on its more advanced and slightly better morphology.

Read More

Are We on the Same Page? A Guide to Time-Lapse Nomenclature and Annotation

Evaluation of embryos in vitro has significantly improved over the past years with the introduction of time-lapse technology, which provides us with new tools to assess the morphological characteristics and implantation potential of an embryo.

Read More

Integrated Microscopes for Esco IVF Workstations

Oocyte pick-up, embryo handling and micromanipulation require various types of microscopes. Most common models of stereo zoom microscopes, transmitted light sources and inverted microscopes can be integrated into Esco IVF Workstations.

Read More

In vitro fertilization (IVF) as Fertility Treatment

The worldwide in vitro fertilization (IVF) services have been projected to show significant growth due to an increase in infertility rate, an inclination of delayed pregnancies, and the rise in IVF success rate.

Read More

IVF Lab Standards, Practices, Accreditation

The Commission of Laboratory Accreditation of the College of American Pathologists (CAP) accredits IVF and andrology laboratories. The CAP is widely considered the leader in quality assurance. CAP accreditation is recognized by the US Federal Government as equal to or more stringent than the government's own inspection program.

Read More